pegPLAND - Plant Prime Editing Guide RNA Design & Structure Visualization

PAM sequence

NGG NG

Cut distance to PAM

Spacer length: 20

Spacer GC content (%): 0-100

Prime editing window (the default values are recommended): 1-15

PBS length: 7-16

PBS GC content (%): 0-100

Recommended Tm of PBS sequence (°C): 30

Homologous RT template length (the default values are recommended): 7-16

Tm-directed PBS length model

Exclude first C in RT template

Dual-pegRNA model

Prime Editor Guide RNA Design