Help & Documentation
Getting Started
Learn the basics of prime editing and how to use pegPLAND for your plant genome editing projects.
- Introduction to Prime Editing
- pegRNA Design Principles
- Plant-specific Considerations
Design Guidelines
Best practices for designing efficient and specific pegRNAs for different edit types.
- PBS Length Optimization
- RT Template Design
- Species-specific Parameters
Structure Analysis
Understanding pegRNA secondary and tertiary structures for optimal design.
- Secondary Structure Prediction
- 3D Molecular Visualization
- Stability Analysis
Troubleshooting
Common issues and solutions for prime editing experiments.
- Low Editing Efficiency
- Off-target Effects
- Design Optimization
Frequently Asked Questions
enpPE2 (enhanced plant prime editor 2) incorporates several optimizations over PE2, including PEmax architecture and engineered pegRNAs with protective motifs, resulting in 2.35- to 29.22-fold increases in mutation frequencies.
Start with 13 nucleotides and test different lengths. PBS with 40-60% GC content typically work best, though sequences outside this range can be optimized.
Engineered pegRNAs (epegRNAs) include structured motifs at their 3' end to protect from degradation. Use them when you need higher stability and efficiency, especially with the evopreQ1 modification.